my account  log out
 

TR-FRET ERR beta Coactivator Assay Kit, goat

Create: 08/11/2017
Expired Date:never
Category: Diagnostic equipment [Health&Medical&Pharmaceutical]
Message:The TR-FRET ERR (estrogen related receptor) beta Coactivator Assay Kit provides a sensitive and robust method for highthroughput screening of potential ERR beta ligands as inverse agonists of ligand-dependent coactivator displacement. The kit uses a terbium (Tb)-labeled anti-GST antibody, a fluorescein-labeled coactivator peptide, and a human recombinant ERR beta ligand-binding domain (ERR beta-LBD) that is tagged with glutathione-S-transferase (GST) in a homogeneous mix-and-read assay format. To assay: When running the ERR beta TR-FRET Coactivator Assay, ERR beta-LBD is added to ligand test compounds followed by addition of a mixture of the fluorescein-coactivator peptide and Tb-labeled anti-GST antibody. After an incubation period at room temperature, the 520 nm/495 nm TR-FRET ratio is calculated and can be used to determine the EC50 from a dose response curve of the compound. Based on the biology of the ERR beta-coactivator peptide interaction, this ligand EC50 is a composite value representing the amount of ligand required to bind to receptor, effect a conformational change, and displace coactivator peptide. http://www.creativebiomart.net/tr-fret-err-beta-coactivator-assay-kit-goat-462781.htm
City:[US] 
Location:
 
May 14 2018TR-FRET ERR alpha Coactivator Assay Kit, goat7
May 14 2018TR-FRET PPAR alpha Coactivator Assay Kit, goat6
May 14 2018Rhod-4 No Wash Calcium Assay kit6
May 14 2018Rhod-4 Medium Removal No Wash Calcium Assay kit7
May 14 2018Calcium Quantification Assay Kit (Luminometric)5
May 14 2018Calcium Quantification Assay kit (Fluorometric)6
May 14 2018Calcium Colorimetric Detection Kit6
May 14 2018Calcium Red Fluorescence Assay Kit6
May 14 2018Calcium Colorimetric Assay Kit6
May 14 2018Calcium Assay Kit6

return back

Copyright © 2012-2017 toextrade Inc. All rights reserved. Contact us