my account  log out

TR-FRET ERR beta Coactivator Assay Kit, goat

Create: 08/11/2017
Expired Date:never
Category: Diagnostic equipment [Health&Medical&Pharmaceutical]
Message:The TR-FRET ERR (estrogen related receptor) beta Coactivator Assay Kit provides a sensitive and robust method for highthroughput screening of potential ERR beta ligands as inverse agonists of ligand-dependent coactivator displacement. The kit uses a terbium (Tb)-labeled anti-GST antibody, a fluorescein-labeled coactivator peptide, and a human recombinant ERR beta ligand-binding domain (ERR beta-LBD) that is tagged with glutathione-S-transferase (GST) in a homogeneous mix-and-read assay format. To assay: When running the ERR beta TR-FRET Coactivator Assay, ERR beta-LBD is added to ligand test compounds followed by addition of a mixture of the fluorescein-coactivator peptide and Tb-labeled anti-GST antibody. After an incubation period at room temperature, the 520 nm/495 nm TR-FRET ratio is calculated and can be used to determine the EC50 from a dose response curve of the compound. Based on the biology of the ERR beta-coactivator peptide interaction, this ligand EC50 is a composite value representing the amount of ligand required to bind to receptor, effect a conformational change, and displace coactivator peptide.
Jan 19 2018PYK2 Kinase Binding Assay Kit2
Jan 19 2018Pyk2 Kinase (Human) Assay/Inhibitor Screening Assay Kit2
Jan 19 2018Pullulanase/Limit-Dextrinase Assay Kit (PULLG6 Method)2
Jan 19 2018 PTP1B Inhibitor Screening Assay Kit2
Jan 19 2018PTP1B Fluorometric Assay Kit2
Jan 19 2018PTP1B drug discovery Kit2
Jan 19 2018 PTP1B Colorimetric Assay Kit2
Jan 19 2018cAMP PTHR2 CHO-K1 GPCR Assay Kit3
Dec 21 2017NMU1R U2OS β-Arrestin GPCR Assay Kit5
Dec 21 2017CYP2C8 Green Screening Kit4

return back

Copyright © 2012-2017 toextrade Inc. All rights reserved. Contact us