my account  log out
 

TR-FRET ERR beta Coactivator Assay Kit, goat

Create: 08/11/2017
Expired Date:never
Category: Diagnostic equipment [Health&Medical&Pharmaceutical]
Message:The TR-FRET ERR (estrogen related receptor) beta Coactivator Assay Kit provides a sensitive and robust method for highthroughput screening of potential ERR beta ligands as inverse agonists of ligand-dependent coactivator displacement. The kit uses a terbium (Tb)-labeled anti-GST antibody, a fluorescein-labeled coactivator peptide, and a human recombinant ERR beta ligand-binding domain (ERR beta-LBD) that is tagged with glutathione-S-transferase (GST) in a homogeneous mix-and-read assay format. To assay: When running the ERR beta TR-FRET Coactivator Assay, ERR beta-LBD is added to ligand test compounds followed by addition of a mixture of the fluorescein-coactivator peptide and Tb-labeled anti-GST antibody. After an incubation period at room temperature, the 520 nm/495 nm TR-FRET ratio is calculated and can be used to determine the EC50 from a dose response curve of the compound. Based on the biology of the ERR beta-coactivator peptide interaction, this ligand EC50 is a composite value representing the amount of ligand required to bind to receptor, effect a conformational change, and displace coactivator peptide. http://www.creativebiomart.net/tr-fret-err-beta-coactivator-assay-kit-goat-462781.htm
City:[US] 
Location:
 
Oct 13 2017MDH Activity Colorimetric Assay Kit13
Oct 13 2017MDA Assay Kit13
Oct 13 2017Lipid Peroxidation (MDA) Colorimetric/Fluorometric Assay Kit12
Oct 13 2017mCXCR7 U2OS β-Arrestin GPCR Assay Kit11
Oct 13 2017mCXCR6 CHO-K1 β-Arrestin GPCR Assay Kit10
Oct 13 2017mCXCR5 CHO-K1 β-Arrestin GPCR Assay Kit10
Oct 13 2017mCXCR4 U2OS β-Arrestin GPCR Assay Kit10
Oct 13 2017mCXCR3 CHO-K1 β-Arrestin GPCR Assay Kit10
Oct 13 2017mCXCR2 CHO-K1 β-Arrestin GPCR Assay Kit10
Oct 13 2017mCX3CR1 CHO-K1 β-Arrestin GPCR Assay Kit9

return back

Copyright © 2012-2017 toextrade Inc. All rights reserved. Contact us