| Message: | This article will introduce an extraction process of heparin sodium, which is simple to operate and has a high yield of heparin sodium, which is suitable for industrialized promotion and use, and specifically includes the following steps:
(1) Raw material processing: clean the fresh small intestine with clean water, scrape the small intestine mucosa after cleaning, and then put the small intestine mucosa in a blender until it becomes minced and set aside;
(2) Heating enzymatic hydrolysis: Put the minced intestinal mucosa obtained in step 1 into a heating tank, add water, mix and stir, and then add 8% trypsin and weak lye to adjust the pH value until the pH value of the solution is 8 -10, and then heat up. During the heating process, the pH should be kept between 8.5-9.5, heated to 30-40°C, kept at a constant temperature for 2-3h, and then continue to heat to 50-60°C, kept at the constant temperature for 10-20min, while Hot filtration to obtain filtrate;
(3) Cooling and adsorption: Cool the filtrate obtained in step 2, and remove the slick oil from the upper layer of the filtrate after cooling to 30-40°C, then add resin to stir and adsorb for 6-7h, and filter out the resin after the adsorption is complete;
(4) Elution: Put the collected resin into the eluent for washing, add 10% sodium chloride solution, keep the temperature at 50-55℃, stir for 3-4 hours, collect the eluate; Elute twice, combine the eluate collected twice for later use;
(5) Precipitation: Filter the eluate collected twice into the precipitation tank, add 80-85% alcohol with mass fraction and stir evenly, then adjust the pH value to keep at 7-8, and let stand for 10-12 hours for sealing and precipitation , Filter to get the precipitate for use;
(6) Dehydration and drying: Put the sediment in a drying oven to obtain heparin sodium.
As a preferred solution, the ratio of water to small intestinal mucosa in step (2) is 1:3. |
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