Message: | Since the outbreak of the epidemic, relevant diagnostic reagent R&D and production companies have introduced RT-PCR nucleic acid detection kits for the first time. By collecting oropharyngeal swab or nasopharyngeal swab samples, the RNA of the virus is extracted and purified for qualitative or quantitative detection. "2019 -New Coronavirus". The sample collection and storage container used in this method is a "disposable virus sampling tube". The new coronavirus nucleic acid detection, disposable virus sampling tube can be said to be indispensable. Next, Desheng intends to introduce the composition and use of the virus sampling tube.
About the composition and manufacturing of the virus sampling tube:
1. Sampling swab: The sampling swab directly contacts the sampling site, and the material of the sampling head is closely related to the subsequent detection. The sampling swab head should be made of Polyester (PE) synthetic fiber or Rayon (man-made fiber), calcium alginate sponge or wooden stick swabs (including bamboo sticks) should not be used, and the swab head should not be made of cotton. Because the fiber of cotton has strong adsorption of protein, it is not easy to be eluted into the subsequent preservation solution; while the wooden sticks or bamboo sticks containing calcium alginate and wooden components will be broken and soaked in the preservation solution will also adsorb protein, even It can inhibit the subsequent PCR reaction.
2. Virus preservation solution: There are two main types of virus preservation solution widely used in the market. One is the improved virus maintenance solution based on the delivery medium, and the other is the improved preservation solution of nucleic acid extraction lysis solution.
3. Preservation tube: The material of the preservation tube should be selected carefully. |