| Message: | Chemiluminescence immuno-CLIA analysis was born in 1977. According to the basic principles of radioimmunoassay, a highly sensitive chemiluminescence technique is combined with a highly specific immune response to establish a chemiluminescence immunoassay. CLIA has the characteristics of high sensitivity, strong specificity, wide linear range, easy operation, and does not require very expensive equipment. CLIA has a wide range of applications. It can detect antigens, haptens and antibodies of different molecular sizes, as well as nucleic acid probes. Compared with radioimmunoassay (RIA), fluorescence immunoassay (IFA) and enzyme immunoassay (EIA), CLIA has the advantages of no radiation, long validity period of markers and full automation.
Chemiluminescence immunoassay is a type of immunoassay method in which antibodies or antigens are directly labeled with chemiluminescence reagents. At present, common markers are mainly luminol and acridine ester chemiluminescent agents.
1 Chemiluminescence immunoassay of luminol label: The luminescence of luminol is oxidation reaction luminescence. In alkaline solutions, luminol can be oxidized to emit light by many oxidants, of which H2O2 is the most commonly used. Due to the slow speed of the luminescence reaction, some enzymes or inorganic catalysts need to be added. The main enzymes are horseradish peroxidase (HRP), and the inorganic ones include O3, halogen, Fe3, Cu2, Co2 and their complexes.
2 Chemiluminescence immunoassay labeled with acridine esters: Acridine esters are used in CLIA because of their poor thermal stability, and a more stable acridinium ester derivative has been synthesized after research. |
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