| Message: | Mops buffer: pH range of 6.5-7.9, can not be autoclaved, pH value changes with temperature to a certain extent, and has high buffer capacity in physiological pH range.
HEPES buffer: pH range is 6.8-8.2, can not be autoclaved, pH value changes with temperature to a certain extent, free radicals will be generated under certain conditions.
Tris buffer: pH range is 7.5-9.0, pH value changes with temperature or dilution degree to a certain extent, which will affect the activity of some enzymes. It has no UV absorption and is cheap.
The function of buffer solution is to adjust the pH value of the solution in a limited range, so that the acidity of the solution to be determined can meet the range specified by the analytical method. For example, phenol can react with 4-aminoantipyrine in alkaline medium and in the presence of potassium ferricyanide to form orange red antipyrine dye. In order to prevent the interference of aromatic amines, the optimum pH is 10 ± 0.2. For this reason, it is necessary to add ammonia chloride buffer solution to the solution to be tested, and adjust and control the pH of the solution to be tested to 10.0 ± 0.2. |
my account
